血清MK和Th17/调节性T细胞对类风湿关节炎患者病情发生发展的影响(1)
[摘要] 目的 觀察辅助性T 细胞17(Th17)/调节性T细胞(Terg)免疫失衡在来风湿关节炎患者发病时的作用，探讨血清中期因子(MK)和Th17/调节性T细胞对类风湿关节炎患者病情发生发展的影响。方法 选取2013年3月～ 2016年1月在我院接受治疗的类风湿关节炎患者58例，分为活动组和非活动组，DAS28≤3.2为非活动组，DAS28>3.2为活动组，另将30例健康患者作为对照组，活动组患者32例，非活动组患者26例，采用酶联免疫吸附法对3组患者的血清MK、白介素-17(IL-17)、白介素-6(IL-6)、白介素-23(IL-23)水平进行检测，利用流式细胞术检测调节性T细胞和外周血Th17细胞的比例变化，并对其比例变化和血清MK、IL-17、IL-6、IL-23的水平进行比较。结果 活动组Th17、调节性T细胞比例分别与非活动组相比较差异具有统计学意义(P<0.05)，活动组与非活动组的Th17、调节性T细胞比例分别与对照组相比较异具有统计学意义(P<0.05)。活动组与非活动组的IL-17、IL-6和IL-23水平分别与对照组相比较异具有统计学意义(P<0.05)。血清MK与Th17、T细胞的百分比和平衡相关的相关性结果显示，Th17和Treg均与血清MK无关，P>0.05，而与STAT5成负相关(P<0.05)。结论 类风湿性关节炎患者的血清MK上升，且类风湿性关节炎患者Th17/Terg平衡中Th17相对增高，而Terg会相对降低，MK的上升和Th17/Terg平衡失调都促进了RA的发生发展。
[关键词] MK；Th17；调节性T细胞；类风湿关节炎
[中图分类号] R593.2 [文献标识码] A [文章编号] 2095-0616(2017)15-107-03
[Abstract] Objective To explore the effect of immune imbalance of T helper cell 17(Th17) and regulatory T cell(Terg) on attack of patients with rheumatoid arthritis and to explore the effect of serum midkine(MK)， Th17 and regulatory T cell on the occurrence and development of patients with rheumatoid arthritis. Methods 58 patients with rheumatoid arthritis who were admitted to our hospital from March 2013 to January 2016 were selected. They were divided into the active group(DAS28>3.2) and the inactive group(DAS28≤3.2). Other 30 healthy people were selected as the control group. There were 32 cases in the active group and 26 cases in the inactive group. Levels of serum MK， Interleukin-17(IL-17)， Interleukin-6(IL-6) and Interleukin-23(IL-23) were detected by enzyme linked immunosorbent assay. Changes of ratio of regulatory T cell and peripheral blood(Th17) were detected by flow cytometry. In addition， changes of ratio and levels of MK， IL-17， IL-6 and IL-23 were compared. Results Th17 and regulatory T cell ratio in the active group and the inactive group had respectively comparative difference and the difference was statistically significant(P<0.05). The active group and the inactive group had respectively comparative differences with the control group in Th17 and regulatory T cell ratio and differences were statistically significant(P<0.05). Levels of IL-17， IL-6 and IL-23 of the active group and the inactive group had respectively comparative differences with the control group and differences were statistically significant(P<0.05). Percentage of serum MK and Th17， percentage of serum MK and T cell and results of correlation related to balance showed that Th17 and Terg were both had no relation with serum MK， P>0.05 while they were negatively correlated with STAT5(P<0.05). Conclusion Serum MK of patients with rheumatoid arthritis increases， Th17 in balance of Th17 and Terg relatively increase and Terg relatively decreases. Increase of MK and imbalance of Th17 and Terg both promote the occurrence and development of RA., 百拇医药(沈聪 刘婵娟 孙宇琦)
[关键词] MK；Th17；调节性T细胞；类风湿关节炎
[中图分类号] R593.2 [文献标识码] A [文章编号] 2095-0616(2017)15-107-03
[Abstract] Objective To explore the effect of immune imbalance of T helper cell 17(Th17) and regulatory T cell(Terg) on attack of patients with rheumatoid arthritis and to explore the effect of serum midkine(MK)， Th17 and regulatory T cell on the occurrence and development of patients with rheumatoid arthritis. Methods 58 patients with rheumatoid arthritis who were admitted to our hospital from March 2013 to January 2016 were selected. They were divided into the active group(DAS28>3.2) and the inactive group(DAS28≤3.2). Other 30 healthy people were selected as the control group. There were 32 cases in the active group and 26 cases in the inactive group. Levels of serum MK， Interleukin-17(IL-17)， Interleukin-6(IL-6) and Interleukin-23(IL-23) were detected by enzyme linked immunosorbent assay. Changes of ratio of regulatory T cell and peripheral blood(Th17) were detected by flow cytometry. In addition， changes of ratio and levels of MK， IL-17， IL-6 and IL-23 were compared. Results Th17 and regulatory T cell ratio in the active group and the inactive group had respectively comparative difference and the difference was statistically significant(P<0.05). The active group and the inactive group had respectively comparative differences with the control group in Th17 and regulatory T cell ratio and differences were statistically significant(P<0.05). Levels of IL-17， IL-6 and IL-23 of the active group and the inactive group had respectively comparative differences with the control group and differences were statistically significant(P<0.05). Percentage of serum MK and Th17， percentage of serum MK and T cell and results of correlation related to balance showed that Th17 and Terg were both had no relation with serum MK， P>0.05 while they were negatively correlated with STAT5(P<0.05). Conclusion Serum MK of patients with rheumatoid arthritis increases， Th17 in balance of Th17 and Terg relatively increase and Terg relatively decreases. Increase of MK and imbalance of Th17 and Terg both promote the occurrence and development of RA., 百拇医药(沈聪 刘婵娟 孙宇琦)